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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be observed in lots of tumors, which leads to chromosome instability, and therefore may perhaps add to tumorigenesis. This protein was identified to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and plays a vital job in promoting mobile-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are already described. Two further splice variants had been noted but thorough nucleotide sequence data just isn't nonetheless out there.
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Blocking peptides are peptides that bind particularly for the focus on antibody and block antibody binding. These peptide generally consists of the epitope recognized via the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to make sure the absolute best results are...
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This guideline outlines further feasible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide not bind towards the epitope within the goal protein. By comparing the staining from the blocked antibody versus the antibody by itself, you can see which staining is precise.
Focus on Data Cyclin E belongs for the very conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and functions as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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